2D-IR spectroscopy shows that optimised DNA minor groove binding of Hoechst33258 follows an induced fit model

Research output: Contribution to journalArticle

The induced fit binding model describes a conformational change occurring when a small molecule binds to its biomacromolecular target. The result is enhanced non-covalent interactions between ligand and biomolecule. Induced fit is well-established for small molecule-protein interactions, but its relevance to small molecule-DNA binding is less clear. We investigate the molecular determinants of Hoechst33258 binding to its preferred A-tract sequence relative to a sub-optimal alternating A-T sequence. Results from 2-dimensional infrared spectroscopy, which is sensitive to H-bonding and molecular structure changes, show that Hoechst33258 binding results in loss of minor groove spine of hydration in both sequences, but an additional perturbation of the base propeller twists occurs in the A-tract binding region. This induced fit maximizes favourable ligand-DNA enthalpic contributions in the optimal binding case and demonstrates that controlling the molecular details that induce subtle changes in DNA structure may hold the key to designing next-generation DNA-binding molecules.
Original languageEnglish
JournalJournal of Physical Chemistry B
Early online date19 Jan 2017
DOIs
StateE-pub ahead of print - 19 Jan 2017

    Research areas

  • spectroscopy, DNA, Hoechst33258, minor groove binding, induced fit, non-covalent interactions, ligand, biomolecule, molecule-DNA binding

Bibliographical note

“This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Physical Chemistry B, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.jpcb.7b00345.

Projects

(1)
  1. EPSRC Doctoral Training Grant - DTA, University of Strathclyde | Hithell, Gordon Robert

    Project: Research Studentship - Internally AllocatedResearch Studentship (Internally Allocated)

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