Temporal imaging of drug dynamics in live cells using stimulated Raman scattering microscopy and a perfusion cell culture system
William Tipping, The University of Strathclyde

This data was generated for the publication of a research article of the same title and investigates the intracellular detection of the discoidin domain receptor 1 inhibitor, 7RH in a panel of cancer cell lines using stimulated Raman scattering microscopy. Raman spectral data was acquired using a Renishaw InVia microscope system controlled using Renishaw WiRE 4.4 software. 

Spectral and imaging datasets are collated as Excel file format (.exe). The data has been post-processed in WiRE 4.4 and plotted on Origin2018, the files are collated in .exe format. SRS images were acquired using a Leica SP8 multiphoton microscope. SRS images are provided in .tif file format with post-processing performed using ImageJ or in .lif format that is compatible with ImageJ.

Figure 1 The raw Raman spectral data is provided in .wdf format compatible with WiRE 4.4 and an .exe file collates the raw and baseline subtracted data. The ratiometric images were produced using Matlab and are provided in .ppt format. The Raw mapping data is provided in .wdf and .spc format that is compatible with Matlab.

Figure 2 The images are provided in .tif file format with scale bars: 10 ?m. A .exe file is provided with quantification data of the 7RH signal/cell at each condition.

Figure 3 The SRS images of the control, 500 nM and 5 ?M 7RH treatments in the four cell lines are provided in .tif format (scale bars: 10 ?m). Quantification data for the drug uptake/cell is provided in .exe format and the cell viability data (AlamarBlue assay) is provided in .exe file format for each cell line.

Figure 4 SRS images of the MCF-7 cells before and during the perfusion experiment in Fig. 4B are provided as separate .tif files. Scale bars: 10 ?m.

Figure 5 The quantification data for the control, 1 ?M and 5 ?M treated cells at 40 mins is provided in .exe format. The data for Fig 5B is provided in .exe format.

Figure 6 The SRS images at each timepoint are collated by timepoint. Scale bars: 10 ?m. The cell viability data is provided as .exe format and the quantification of 7RH uptake concentration/cell is also in .exe format for each timepoint presented in A.

Figure 7 SRS images for the adhesion assay in both MCF-7 and MDA-MB-231 cells are provided in .tif format with scale bars: 10 ?m. Quantification data for the number of adherent cells is provided in .exe format. The SRS images of the scratch-wound assays are provided in .tif format. Scale bars: 100 ?m. Quantification data is provided in .exe format. 

Figure S1 SRS images presented in Fig. S1 are provided in .tif format with scale bars: 10 ?m.

Figure S2 The SRS presented in Fig. S1 are provided in .tif format with scale bars: 10 ?m. Quantification data is provided in .exe format. 

Figure S4 The perfusion images are provided in .tif format. Images are acquired in SRS or fluorescence mode.

Figure S5 The perfusion images are provided in .tif format. Images are acquired in SRS or fluorescence mode.


Figure S6 The perfusion images are provided in .tif format. Images are acquired in SRS or fluorescence mode.