The data are sorted in folders after the dye (DAPI, Hoechst, Calcofluor White, Alexa 350, SYTOXGreen) and the type of data (spectrum, photobeaching data, power dependence) with an indication of which figure in the paper they belong to.
The folder figure1 are the two images in figure 1.


Photobleaching data:
Each subfolder, named with the wavelength and excitation power used, contains a series of images (ome.tiff - can be opened in ImageJ) from a continuous acquisition.

There is also a file (text file) containing the measured laser power during the aqcuisition.


Power dependence:
Files are named with the excitation power. Files marked "after" are acquired after the rest of the data, to check for photobleaching effects.



Spectra:
The files are named with the excitation power if that varied over the series.
To reduce the effect of photobleaching, the data is acquired in overlapping sets, with only a few excitation wavelengths per area. The files are named according to
"675nm21mW22.ome.tif" = Excitation at 675 nm, with 21mW, area 2 measurement no. 2 (measurements 1 and 3 are repeats of the same datapoint (excitation wavelength) to check for photobleaching and allow concatenation of datasets)
For the data analysis and figures the data are corrected for the excitation power.


ALL DATA ARE LABELLED WITH THE POWER AT MICROSCOPE INPUT, rather than the power at the sample plane (which is obviously what we discuss in the paper).

The power can be converted using the measured objective transmission: see file "ExcitationPowersReal.xlsx" (also available as a pdf "print-out" for backwards compatibility with MSOffice )

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These data have not previously been published.