All images are binary files, 16 bit, little endian byte order. Images are created by a Labview program controlling the microscope that I wrote.

Each image is named "Filename_Dim_500x500_Int16.bin", where 500x500 is the dimension (number of pixels) in x and y.

The accompanying file "Filename_Info" contains information about the date of acqusition, scaling of the pixels, averaging, scan speed. Calculate the real scale as Xdim(um)=[Fast Axis Amplitude]*[Scaling um/V], Ydim(um)=[Slow Axis Max-Slow Axis Min]*[Scaling um/V]. This is correct for a 40X objective. (For data taken with a 60X objective (indicated below or in manuscript text) scale with 2/3)

All images have a software added offset of 100 counts.

For images of specimens stained with two fluorophores, the two images are acquired sequentially with the same PMT gain setting.

The laser powers indicated in the file names are the powers at the input of the microscope (NOT at the sample plane)

Files named "bg_Dim_500x500_Int16.bin" are images with the laser blocked to allow for background correction.

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DATA ASSOCIATED WITH FIGURES:

Figure 2:
PowerDependenceAndSpectra.xlsx

Figure 3:
50mw_4j_Dim_500x500_Int16.bin


Figure 4:
a)
obj22sameAreaAsobj1_Dim_500x500_Int16.bin

b)
fluocell2_1130nm_72mW_Dim_500x500_Int16.bin

c)
Excitation residual pump:
Kidney_840_60mwGFPfilter_Dim_1000x1000_Int16.bin

Excitation Raman laser:
Kidney_1080_50mw_Dim_1000x1000_Int16.bin


DATA ASSOCIATED WITH STATEMENTS:

1) Two photon power dependence:
PowerDependence.zip
Files named "FilenameAfter" are acquired at the end of the series to check for photobleaching.

2) Comparison of exitation with OPO and Raman laser
CompareOPORaman.zip

3) Imaging a sample stained with Alexa 594
Alexa594Pdep.zip

4) Imaging a sample stained with dsRed
dsRed.zip
(files are named with the pump wavelenth used, 850nm=excitation wavelength=1110nm