All image data in binary format are 16 bit signed and has Little endian byte order. Naming convention: "Filename_Dim_500x200_Int16.bin" Image is 500 pixels high (n.o. scan lines) and 200 pixels wide. Each image file has an associated text format info file "Filename_Info.txt", with information on scan frequency and scaling information (Volts applied to galvo mirrors and microns/V in image plane (44.140 um/V which is correct for the images taken with a 40X objective (data on spirogyra, Elodea leaf). For data taken with 60X objective (RFP expressing cells, primrose leaf hair, Zresolution on beads) multiply by 2/3 ) For data labelled with a power (power series, comparison OPO_SPRG the power is measured at the microscope input. For data labelled with a position (z-stacks) the z-value should be multiplied by 3.55 to get the corecct scaling (calibration factor for the PiezoJena Z-stage) ======================================= 1) Spectra of the SPRG 1100LPOldXtalBetter.txt Spectrum with 100nm short pass filter. Suppl. fig S3 2 column txt file with header PumpPowerDependenceSpectrum.txt Spectra at different pump powers. Fig 1b,e, Fig S2a 2 column txt file with header -------------------------------------------- 2) Output signal pump power dependence PumpPowerDependence.txt Fig S2b 2 column txt file with header ---------------------------------------------- 3) Autocorrelator traces ACtrace.txt (plottable data) ACtrace_file1.txt (raw data from AC, 2 column txt file with header) -------------------------------------------- 4) Beam profile camera image Beamprofile1.bmp -------------------------------------------- 5) Z resolution measurement Folder: ZResolution_TetraSpecBeads.zip Z stack of images through a bead sample Subfolder Pdep: power dependence of the signal -------------------------------------------- 6) Two photon imaging of RFP expressing HeLa cells Folder: Twophoton_imaging_RFP.zip b12mW60x_Dim_1000x1000_Int16.bin Figure 2a folder PowerDependence Power dependence of signal folder Compare1170_1064 Comparison of signal level with SPRG and pump laser -------------------------------------------- 7) THG imaging of Spirogyra using SPRG folder THG_imaging_Spirogyra Contains: the power dependence of the signal (2 folders) the z-stack in figure 2b -------------------------------------------- 8) Measurements of the noise form the Raman Generator folder: noise 2 column txt file no header comma delimited time (s), signal data from oscilloscope -------------------------------------------- 9) Cytoplamic streaming in Elodea folder: elodea 37 sequential THG images of Elodea leaf. Figure 3 10) Cytoplamic streaming in Primrose folder: Primrose Bright field video. Avi format: t22min.avi THG image of the same area "thg0_Dim_500x500_Int16.bin" Suppl fig S5 -------------------------------------------- 11) Comparison of the THG signal intensity with OPO and SPRG Data for supplementary figure S4 11)CompOPO_SPRG