Raman Repeat 1 contains all raw Raman measurements (maps) for the first biological replicate in .wdf format in addition to measurements post processing in Renishaw Wire 4.1 in .wdf format (with the suffix ‘Copy’ and measurements post processing in Renishaw Wire 4.1 in .spc format (for import into Matlab). Raman Repeat 2 contains all raw Raman measurements (maps) for the second biological replicate in .wdf format in addition to measurements post processing in Renishaw Wire 4.1 in .wdf format (with the suffix ‘Copy’ and measurements post processing in Renishaw Wire 4.1 in .spc format (for import into Matlab). Raman Repeat 3 contains all raw Raman measurements (maps) for the third biological replicate in .wdf format in addition to measurements post processing in Renishaw Wire 4.1 in .wdf format (with the suffix ‘Copy’ and measurements post processing in Renishaw Wire 4.1 in .spc format (for import into Matlab). 07042017 Live Dead staining contains all raw images of cells stained for viability in .tif format and post processing (‘Processed’) in .jpg format along with a Word file with LUT values for the processed images. Fluorescence 30033017 contains the raw .lif data file for phospholipidosis and steatosis staining as well as exported .jpg images (‘Images’) in the format of individual channels, overlayed channels and overlayed channels with brightfield.